Automated early toxicity profiling

toxicity ASSESSMENT HAS NEVER BEEN THAT fast

GET WHOLE-ORGANISM DATA WITHOUT THE USE OF VERTEBRATES.

1 week. 1 experiment.

Toxicity assessment using traditional animal testing can be expensive, time-consuming and subjected to ethical concerns. On the other hand, the use of cell-based models as an alternative for early toxicology tests does not deliver physiological responses and multi-organ crosstalk data.

Bridge the gap between vertebrates and cell models seamlessly with Nagi products. Fast and easy early toxicology assays.

SOT and ToxExpo 2024 Salt Lake City Nagi Bioscience DART early toxicology

Nagi early Toxicology Assays

REPROductive TOXicology ASSAY

reproductive toxicology assay

NAGI™ REPROTOX ASSAY

The assay to study the potential effects of your molecules on the reproductive capacity using the model organism C. elegans.

Method Description

reproductive toxicology assay description

Readouts

Maternal effect readouts

  • Worm lethality
  • Worm size
  • Growth dynamics
  • Sexual maturity
  • Worm shape

Reprotox Readouts

  • Fertility
  • Embryonic viability
  • Progeny accumulation
C. elegans reproduction over 96 hours post-hatching for wild-type N2 worms treated with the anticancer drug 5-fluorouracil starting from the L4 larval stage (right) and untreated worms (left) as observed via brightfield imaging by the SydLab™ One. Overlay masks for the detection of worms and eggs are automatically generated by SydLab’s AI-based computer vision algorithm.

Check the data

5-FU reproductive toxicity assay results

Temporal evolution of the average number of larvae produced by wild-type N2 C. elegans treated with the anticancer drug 5-fluorouracil (5-FU) starting from the L4 larval stage vs untreated worms (vehicle). The drug treatment significantly impacts on C.elegans reproductive process. Data provided by SydLab™ One.

reproduction dose response c elegans

Progeny production rate over the first 24 hours of reproduction for wild-type N2 C. elegans treated with the anticancer drug 5-fluorouracil (5-FU) at different doses (data normalized to the value measured for the untreated worm population). 5-fluorouracil treatment specifically affects the worm reproduction, inducing strong embryotoxicity. Data provided by SydLab™ One.

DEVELOPMENTAL TOXICITY ASSAY

developmental toxicity assay

NAGI™ DEVTOX ASSAY

The assay to explore the potential adverse effects of your molecules on the development of C. elegans worms. It can be used as a prediction for teratogenic potency of the test compounds, among other possibilities.

METHOD DESCRIPTION

developmental toxicology assay description

READOUTS

  • Worm lethality
  • Worm size
  • Growth dynamics
  • Sexual maturity
  • Worm shape
  • Fertility
  • Embryonic viability
  • Progeny accumulation rate
C. elegans development over 96 hours post-hatching for wild-type N2 worms treated with the antibiotic doxycycline, starting from the L1 larval stage (right) and untreated worms (left), as observed via brightfield imaging by the SydLab™ One.

Check the data

development curve toxicity assay

Temporal evolution of the average worm size for wild-type N2 C. elegans treated with the antibiotic doxycycline starting from the L1 larval stage vs untreated worms (vehicle). The drug treatment significantly impacts on C. elegans development, size and growth rate. Data provided by SydLab™ One.

development dose response antibiotic doxycycline

Average time to reach half of the maximal size for wild-type N2 C. elegans treated with the antibiotic doxycycline at different doses (data normalized to the value measured for the untreated worm population). Doxycycline treatment significantly impacts on worm development, resulting in growth retardation and reduced size. Data provided by SydLab™ One.

C. elegans worms fluorescence assay

Use Case

AUTOMATION OF EARLY TOXICITY PROFILING USING C. ELEGANS NEMATODES
Automation of early toxicity profiling

C. ELEGANS FOR PREDICTIVE TOXICOLOGY

  • C. elegans is a well-studied model with useful features for rapid investigations due to its physiological characteristics.
  • Many pathways important in development, such as reproduction and modes of toxic action, are conserved.
  • Concordant response areas include growth, development, LD50 ranking and neurotoxicity.

Ref: Hunt et al. (US FDA), 2020

c elegans model organism advantages and characteristics

“C. elegans can provide a bridge between in vitro assays and mammalian toxicity testing (…), although standardized culture practices are required to achieve consistent results”

Hunt et al. (US FDA), 2020

LEARN MORE ABOUT OUR BIOLOGICAL MODELS

providing for the first time high STANDARDIZATION & reproducibility levels

to make small organisms toxicology testing easy

welcome to the sydlab™ one universe

Nagi experiment kits
SydLab One C. elegans automation
Software solutions for the SydLab System