A synchronized population of C. elegans is injected into the microfluidic platform at the first larval stage (L1). Worms are confined within dedicated microfluidic chambers and are continuously fed with an E. coli solution.
Worms are chronically exposed to the test compounds right after the injection (L1 stage) for 130 hours (day 3 of adulthood). The test compounds are mixed with the E. coli solution. Freeze-dried OP50 E. coli are used as a food source, preventing the metabolization of the tested molecules by the bacteria.
The images of each microfluidic chamber are recorded every hour. Time-resolved phenotypic readouts are then extracted from the collected images.
- Worm lethality
- Worm size
- Growth dynamics
- Sexual maturity
- Embryonic viability
- Progeny accumulation rate