A synchronized population of C. elegans is injected into the microfluidic platform at the first larval stage (L1). Worms are then confined within dedicated microfluidic chambers and are continuously fed with an E. coli solution.
Worms are chronically exposed to the test compounds starting from the last larval stage prior to sexual maturity (L4) for 85 hours (day 3 of adulthood). The compounds to be tested are mixed with the E. coli solution. Freeze-dried OP50 E. coli are used as a food source for the whole duration of the experiment, preventing the metabolization of the tested molecules by the bacteria.
Starting from 70 hours post-worm injection (day 1 of adulthood), short video sequences are recorded every six hours for each microfluidic chamber. Time-resolved readouts are then extracted from the acquired videos for a diversified characterization of the worms’ motility.
- Bending frequency
- Amplitude of the movement of the head
- Amplitude of the movement at the middle of the body
- Amplitude of the movement of the tail