UNLOCK THE full POTENTIAL OF C. elegans to empower YOUr research

Unique datapoints at an unprecedented reproducibility

UNDERSTANDING LONGEVITY REGULATION

The biological model C. elegans has been fundamental in aging and longevity research allowing groundbreaking findings about human lifespan, such as Insulin/Insulin-like Growth Factor (IGF-1) signaling pathway or the discovery of the daf-2 gene.

From motility phenotypes to molecular neurodegenration, perform any C. elegans assay seamlessly with our technologies

SCREEN YOUR
ANTI-AGING CANDIDATES

C. elegans nematodes are an ideal model organism for the screening of aging-related drug candidates due to its short lifespan (~15 days), tractable genetics and the conservation of essential age-related metabolic pathways between C. elegans and humans.

High-throughput and high-content screening of your candidates is finally possible thanks to SydLab™ One, making C. elegans research fast and easy.

EXPLORE THE EFFECTS OF TEST COMPOUNDS ON LIFESPAN & HEALTHSPAN

Manual protocols can hinder your research by its inability to obtain important readouts from micro-organisms such as nematodes. Unlock unique lifespan and healthspan datapoints by automating your full experimentation process in just a click

Our pioneer technology allows you to easily design and run experiments so you can focus on what really matters: your results.

C. ELEGANS: A POWERFUL MODEL FOR LONGEVITY STUDIES

  • Widely used for more than 60 years to address fundamental aspects of longevity and aging research, such as metabolic diseases and neurodegenerative illnesses.
  • Conservation of essential age-related metabolic pathways between C. elegans and humans.
  • Scalable, faster and ethical alternative to animal testing.
Publications on PubMed about aging diseases per model organism
Publications on PubMed with keyword “Longevity” per model organism (Blue=all time ; green=2010-2020).

LEARN MORE ABOUT OUR BIOLOGICAL MODELS

Nagi aging research Assays

LIFESPAN PHENOTYPE DETECTION ASSAY

lifespan aging assay

NAGI™ LIFESPAN ASSAY

The assay is designed to understand longevity regulation, explore the effects of test compounds on aging, and screen for anti-aging candidates. Bring your research to the next level with unique datapoints.

Method Description

Aging research assay timeline

Readouts

  • Growth parameters
  • Reproductive parameters
  • Mean & maximum lifespan
  • Survival
  • Healthspan (motility)
Lifespan: 22 days
Lifespan: 17 days

Check the data

Worm motility

motility phenotypes readouts

With our analysis software we can extract a multi-phenotypic fingerprint plot including readouts of body bends frequency, velocity, and curvature, as well as amplitude of movement in the head, tail and middle of the body. Data obtained by SydLab™ One.

Automated survival analysis

survival rate graph c elegans

The worm survival curve is extracted automatically with a 6-hour time resolution, based on the Kaplan-Meier model. The mean lifespan values obtained with our microfluidic platform are therefore consistent with the published literature data. Data obtained by SydLab™ One.

MOTILITY PHENOTYPES DETECTION

motility assay

NAGI MOTILITY PHENOTYPES DETeCTION

The assay is designed to evaluate the effects of test compounds on C. elegans behavior and motility, as well as to provide indications about neurotoxicity, muscular toxicity or premature aging process.

MOTILITY AND BEHAVIOR

NEUROTOXICITY assessment

MUSCULAR TOXICITY

Premature Aging

METHOD DESCRIPTION

READOUTS

  • Bending frequency
  • Velocity
  • Amplitude of the movement of the head
  • Amplitude of the movementat the middle of the body
  • Amplitude of the movement of the tail
  • Curvature
Movement analysis for a wild-type worm during high (left) and low (right) physical activity. Video and ML-based analysis automatically generated by the SydLab™ motility analysis software module.

Check the data

Motility high activity

Representative multi-phenotypic fingerprint plot comparing a control worm and a more active worm at day 1 of adulthood, which includes readouts of body bends frequency, velocity, curvature as well as amplitude of the movement at the head, tail and middle of the body. In conclusion, this high-content motion analysis reveals very subtle changes in C. elegans movements, beyond average motility measurements. Data obtained by SydLab™ One.

Motility low activity

Representative multi-phenotypic fingerprint plot comparing a control worm with a less active worm at day 1 of adulthood, which includes readouts of body bends frequency, velocity, curvature as well as amplitude of the movement at the head, tail and middle of the body. In this case, this high-content motion analysis reveals very subtle changes in C. elegans movements, beyond average motility measurements. Data obtained by SydLab™ One.

UNIQUE RESEARCH NEEDS VERSATILITY

COMBINE THE MOTILITY FEATURES OF SYDLABtm One WITH OTHER ASSAYS
lifespan aging assay

LIfespan phenotype detection assay

neurodegeneration aging research assay

Neurodegeneration assays

developmental toxicity assay

developmental toxicity assay

MOLECULAR NEURODEGENeRATION ASSAY

neurodegeneration aging research assay

Study of worm disease models

The assay to monitor the effects of test compounds on aggregate formation and aggregation dynamics in numerous C. elegans models of neurodegenerative diseases.

  • Worms model of Amyotrophic Lateral Sclerosis (ALS)​
  • In vivo monitoring of the hSOD1 aggregates​
  • Quantification of the size and number of the aggregates during aging​

METHOD DESCRIPTION

  • For this assay we take advantage of the existing C. elegans GFP-reporter strains, modelling a variety of neurodegenerative disorders, such as Alzheimer’s, Parkinson’s, Huntington’s disease and amyotrophic lateral sclerosis (ALS), as well as the capacity of SydLab™ One to perform fluorescent imaging. 
  • A synchronized population of C. elegans is automatically injected into the Nagi™ Chips thanks to SydLab™ One at the first larval stage (L1). Worms are then confined within dedicated microfluidic chambers of the Nagi™ Chips and are continuously fed with an E. coli solution. 
  • Worms are chronically exposed to the test compounds starting from the last larval stage prior to sexual maturity (L4) for 85 hours (day 3 of adulthood). The entire process of culture and treatment administration is handled by SydLab™ One. The compounds to be tested are mixed with the E. coli solution fed to the worms. Freeze-dried OP50 E. coli are used as a food source for the whole duration of the experiment, preventing the metabolization of the tested molecules by the bacteria.
  • The images of each microfluidic chamber are recorded every hour by SydLab™ One. Starting from 70 hours post worm injection (day 1 of adulthood), both brightfield and fluorescent images start to be recorded in order to monitor worm growth and protein aggregation dynamics in parallel. Time-resolved phenotypic readouts are then extracted from the collected images in both brightfield and fluorescent modes.

Check the data

neurodegeneration data
Temporal evolution of the average number of polyQ::yfp aggregates counted per each worm of two different strains: AM140 (C. elegans model for human Huntington’s disease) and AM134 (control strain). “Humanized” C. elegans strains modeling all major neurodegenerative disorders exist, also including e.g. ALS, Alzheimer’s, and Parkinson’s disease. Data obtained by SydLab™ One.

CASE STUDIES: Aging Research

DATA IS WORTH THOUSAND WORDS
ANTI-AGING DRUG VALIDATION
DIETARY RESTRICTION
Automation of aging research ON C. ELEGANS

“THE INTEGRATION OF NAGI’S TECHNOLOGY OPENED MANY POSSIBILITIES FOR US.”

THE QUEST for BEST CANDIDATES IDENTIFICATIOn

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Nagi experiment kits
SydLab One C. elegans automation
Software solutions for the SydLab System