Alzheimer’s disease (AD), the most common form of dementia, is an age-related disease strongly associated with mitochondrial dysfunction and metabolic imbalance, both of which accelerate cellular and neuronal decline. Mitochondrial dysfunction, such as mitochondrial hypermetabolism, has been identified as a critical event in the onset and progression of AD. In parallel, caloric restriction (CR) stands…
Background While many biological studies can be performed on cell-based systems, the investigation of molecular pathways related to complex human dysfunctions – e.g. neurodegenerative diseases – often requires long-term studies in animal models. The nematode Caenorhabditis elegans represents one of the best model organisms for many of these tests and, therefore, versatile and automated systems for accurate…
Phosphorylation of the N-terminal domain of the huntingtin (HTT) protein has emerged as an important regulator of its localization, structure, aggregation, clearance and toxicity. However, validation of the effect of bona fide phosphorylation in vivo and assessing the therapeutic potential of targeting phosphorylation for the treatment of Huntington’s disease (HD) require the identification of the…