What you’ll discover inside the guide: Why C. elegans is a powerful whole-organism model for early toxicology. How the Nagi DART assay reached 85% balanced accuracy across a 21 benchmark chemicals study. Real examples of compounds like Paraquat and 5-FU and the insights uncovered. How multi-endpoint readouts speed up decision-making while reducing vertebrate testing by…
Conventional toxicological assays have limitations: cellular models lack organismal complexity, and vertebrate testing is costly, ethically constrained, and low throughput. Caenorhabditis elegans provides a promising alternative, enabling whole-organism testing at an in vitro-like scale with easier handling and lower costs. To enhance scalability, we developed SydLab™ One, an automated microfluidic platform for culturing, treating, and…
Nematode Caenorhabditis elegans constitutes a valuable NAMs model for multiple applications, including predictive toxicology. This microscopic worm gained popularity for its ideal short size and life cycle, ease of cultivation and propagation, and powerful genetic toolkit. While C. elegans has the potential to complement in vitro models to better predict toxic outcomes in mammals, the…
Currently existing biological testing strategies still involve an extensive animal experimentation in vertebrate models, which is expensive and is associated with a number of important ethical concerns and regulatory constraints. The alternative methods to animal testing are typically based on cellular models. The main limitation of these in vitro techniques is that they cannot predict…
We propose an innovative solution for rapid identification of toxic com- pounds and their potential mechanism of toxicity, using a biological model that perfectly bridges the gap between in vitro and in vivo assays. Our technology allows not only to perform endpoint measurements, but to monitor the dynamics of biological responses.
Background and Objectives Nematode Caenorhabditis elegans constitutes a valuable NAMs model for multiple applications, including predictive toxicology. This microscopic worm gained popularity for its ideal short size and life cycle, ease of cultivation and propagation, and powerful genetic toolkit. While C. elegans has the potential to complement in vitro models to better predict toxic outcomes…
Background and Objectives Nematode Caenorhabditis elegans constitutes a valuable NAMs model for multiple applications, including predictive toxicology. This microscopic worm gained popularity for its ideal short size and life cycle, ease of cultivation and propagation, and powerful genetic toolkit. While C. elegans has the potential to complement in vitro models to better predict toxic outcomes…
We describe an innovative platform for fully automated handling and observation of C. elegans, combined with dedicated software for data collection and analysis. Our microfluidic device allows, for the first time, automated high-content phenotyping of worms at medium/high-throughput, via accurate control and real-time monitoring of multiple physiological parameters in the worms. This screening format significantly minimizes the amount…
Thanks to its tiny size (1-mm long), the nematode Caenorhabditis elegans can easily fit into a microfluidic chip, offering a new alternative for animal testing and at the in vitro scale. C. elegans worms have been widely used for studying development, stress, and aging and have been recently used in environmental toxicology studies. The other…