Here we describe an innovative platform which combines the use of the nematode Caenorhabditis elegans, as validated in vivo model for compound screening, with the first laboratory device for its fully automated in vitro culture, treatment and multi-parametric analysis. Our microfluidic device allows automated high-content phenotyping of C. elegans, via accurate control of worm culture conditions and real-time automated…
Phosphorylation of the N-terminal domain of the huntingtin (HTT) protein has emerged as an important regulator of its localization, structure, aggregation, clearance and toxicity. However, validation of the effect of bona fide phosphorylation in vivo and assessing the therapeutic potential of targeting phosphorylation for the treatment of Huntington’s disease (HD) require the identification of the…
Currently existing toxicology testing still implies an extensive experimentation in mammalian models, which is expensive and associated with important ethical concerns. The alternative methods to animal testing are typically based on cellular models. The main limitation of these in vitro approaches is that they often cannot predict complex responses at the level of an organism, usually involving…